Learn Genome Assembly and Annotation in Prokaryotes and Eukaryotes

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Learn Genome Assembly and Annotation in Prokaryotes and Eukaryotes

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About Course

Learn Step by Step Genome Assembly & Annotation in Prokaryotes and Eukaryotes

Turn raw DNA sequencing data into meaningful biological insights. In this practical, hands-on bioinformatics course, you’ll learn how to perform genome assembly and annotation for both prokaryotic and eukaryotic organisms using real datasets and powerful open-source tools.

Whether you’re a student, researcher, or bioinformatics enthusiast, this course will help you master skills used in genomics, biotechnology, medicine, and evolutionary biology. No prior programming experience is required. We start from the basics and guide you through each step.

What You’ll Learn

  • Perform quality control and preprocessing of sequencing reads

  • Assemble genomes with tools like SPAdes and Flye

  • Annotate genes using Prokka and AUGUSTUS

  • Add functional data with BLAST and Pfam

  • Visualize genomes using Quast

  • Build reproducible pipelines with Bash scripting

  • Work confidently with both prokaryotic and eukaryotic datasets

Who This Course Is For

  • Biology and bioinformatics students

  • Researchers and lab technicians

  • Educators teaching genomics hands-on

  • Self-learners curious about genome analysis

What You Need

Just a computer, internet connection, and basic biology knowledge. We’ll guide you through setting up your bioinformatics environment and provide downloadable scripts for practice.

By the end of this course, you’ll be able to take raw sequencing reads, assemble a genome, annotate it, visualize results, and prepare them for publication or database submission.

Enroll now and start your journey into genome assembly and annotation turning data into discovery.

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What Will You Learn?

  • Understand the biological principles behind genome assembly and annotation.
  • Set up a Linux-based bioinformatics environment, including WSL on Windows.
  • Download and manage raw sequencing data from public repositories like NCBI and ENA.
  • Perform quality control and trimming of sequencing reads using FastQC and fastp.
  • Assemble genomes using SPAdes and Flye.
  • Annotate prokaryotic genomes using Prokka.
  • Annotate Eukaryotic Genomes using AUGUSTUS.
  • Run BLAST for functional annotation and pathway mapping.
  • Predict protein domains and GO terms using HMMER and Pfam databases.
  • Visualize and validate annotation results using Quast.
  • Build reproducible genome assembly and annotation pipelines using Bash scripting.

Course Content

Course Introduction

  • Course Introduction
    06:14

Linux for Bioinformatics

Short Read Genome Assembly and Analysis Using Linux

Long Read Genome Assembly and Analysis Using Linux

Prokaryotic Genome Annotation

Eukaryotic Genome Annotation

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Your Instructor

Abdul Rehman Ikram

Bioinformatician | Data Analyst | Computational Biologist

Abdul is a distinguished bioinformatician, data analyst, and computational biologist known for his exceptional contributions to the field of biomedical research. With a passion for integrating technology and biology, Abdul has carved a niche for himself, leveraging cutting-edge computational techniques to unravel complex biological data.

Driven by a curiosity to decode the complexities of life, Abdul believes in the power of interdisciplinary approaches. He is committed to mentoring the next generation of scientists, fostering a culture of innovation and continuous learning.

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