Learn Antimicrobial Resistance Detection (AMR) Analysis using Linux

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Learn Antimicrobial Resistance Detection (AMR) Analysis using Linux

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About Course

Antimicrobial resistance (AMR) is one of the most critical challenges in modern medicine and bioinformatics provides the tools to detect, analyze, and predict resistance directly from genomic data.

In this hands-on course, you’ll learn how to build complete AMR analysis pipelines starting from raw sequencing reads all the way to machine learning-based resistance prediction.

You’ll begin with the fundamentals of AMR and bioinformatics, then move on to Linux essentials, data preprocessing, and genome assembly using tools like SPAdes and Quast. Next, you’ll perform genome annotation with Prokka and detect resistance genes through ABRicate using multiple AMR databases (CARD, NCBI, ResFinder).

Finally, you’ll learn how to extract key features from AMR data, build an AMR gene presence–absence matrix, and apply machine learning models in Python to predict resistance patterns.

This course combines real-world genomic data, practical coding, and clear explanations to help you master AMR genomics analysis even if you’re a beginner.

No coding is required: all pipelines and codes are provided! Just follow the guided workflow and focus on learning the biological insights.

By the end of this course, you will:

  • Understand the principles of antimicrobial resistance genomics

  • Perform quality control and genome assembly using Linux-based tools

  • Annotate genomes and detect AMR genes using Prokka and ABRicate

  • Utilize major AMR databases for gene identification

  • Prepare AMR gene presence–absence data for ML analysis

  • Apply machine learning models to predict resistance patterns

  • Use fully provided codes and pipelines without manual scripting

Ideal For:

  • Students and researchers in bioinformatics, genomics, and microbiology

  • Beginners who want a guided, no-coding approach to AMR analysis

  • Professionals seeking hands-on AMR detection pipelines for real data

  • Anyone curious about integrating bioinformatics and machine learning

Enroll now and start your journey to master AMR genomics and machine learning powered resistance detection today!

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What Will You Learn?

  • Understand the fundamentals of Antimicrobial Resistance (AMR) and its biological significance.
  • Learn how bioinformatics tools and databases are applied in AMR research and genomic analysis.
  • Set up a Linux-based bioinformatics environment and efficiently navigate the Linux file system.
  • Perform data preprocessing and quality control using tools like FastQC and Fastp.
  • Conduct de novo bacterial genome assembly using SPAdes and assess assembly quality with Quast.
  • Annotate genomes using Prokka and interpret gene annotation results in the context of AMR research.
  • Detect antimicrobial resistance genes from multiple databases using ABRicate.
  • Integrate all steps into a complete AMR analysis pipeline from raw data to gene detection.
  • Generate an AMR gene presence–absence matrix and prepare data for downstream analysis using Python.
  • Build and interpret machine learning models to predict antimicrobial resistance patterns based on genomic data.

Course Content

Introduction

  • What is Antimicrobial Resistance (AMR)?
    15:41
  • Bioinformatics in AMR Research
    20:03
  • Overview of the Course Pipelines
    25:44
  • Setting Up Your Environment and Downloading Raw Data
    29:53
  • Assignment 1: Install Tools and Run a Simple Bash Script Test

Basic Linux For Bioinformatics (Optional)

Data Preparation and Quality Control

Genome Assembly

Genome Annotation

AMR Gene Detection and Analysis

Advanced Machine Learning Models and Interpretation for AMR Genes

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Your Instructor

Abdul Rehman Ikram

Bioinformatician | Data Analyst | Computational Biologist

Abdul is a distinguished bioinformatician, data analyst, and computational biologist known for his exceptional contributions to the field of biomedical research. With a passion for integrating technology and biology, Abdul has carved a niche for himself, leveraging cutting-edge computational techniques to unravel complex biological data.

Driven by a curiosity to decode the complexities of life, Abdul believes in the power of interdisciplinary approaches. He is committed to mentoring the next generation of scientists, fostering a culture of innovation and continuous learning.

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